Fibroblast cultures of nude mouse skin as targets for transformation by chemical carcinogens

Laaksonen, A.M.; Mäntyjärvi, R.A.; Hänninen, O.O.

Medical Biology 61(1): 59-64


ISSN/ISBN: 0302-2137
PMID: 6302407
Accession: 005459334

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Secondary cultures of newborn NMRI nu/nu (nude) mouse fibroblasts were used as targets for transformation by chemical carcinogen. 3-Methylcholanthrene, benzo(a)pyrene and benz(a)anthracene induced the development of foci of transformed cells in cultures, whereas no foci were obtained under similar conditions when the non-carcinogenic chenical anthracene was used. The frequency of focus formation was dependent on cell density and on the chemical and its concentration. The frequency of focus formation by 3-methylcholanthrene, the most potent compound tested, ranged up to 22 foci per 105 surviving cells, whereas benz(a)anthracene induced 1.8 foci under similar conditions. A high level of aryl hydrocarbon hydroxylase, a carcinogen-activating enzyme complex, was detected in the fibroblast cultures used.