Formation of putrescine and cinnamoyl putrescines in tobacco nicotiana tabacum cultivar xanthi cell cultures

Berlin, J.

Phytochemistry (Oxford) 20(1): 53-56

1981


ISSN/ISBN: 0031-9422
Accession: 005481207

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Abstract
A p-fluorophenylalanine-(PFP) resistant cell line [TX-4] of N. tabacum and wild type cells [TX-1] accumulating high and low levels of cinnamoyl putrescines, respectively, were used to study the formation of putrescine in the biosynthesis of cinnamoyl putrescines. Labeled arginine and ornithine were equally well incorporated into the main conjugates caffeoyl and feruloyl putrescine. Trapping experiments indicated that both amino acids were decarboxylated for putrescine biosynthesis. Nearly all alcohol-extractable radioactivity from the labelled amino acids was found as cinnamoyl putrescines in the PFP-resistant cell line; wild type cells retained significant radioactivity in the amino acids. The enzyme activities of arginine and ornithine decarboxylases in the resistant cell line were increased 3- to 6-fold.