EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Forms and activity of high molecular weight acth in guinea pig


Forms and activity of high molecular weight acth in guinea pig



Peptides 1(3): 223-230



ISSN/ISBN: 0196-9781

High MW forms of ACTH and endorphin were identified in extracts of guinea pig anterior and inermediate/posterior pituitary. Extracts of anterior pituitary contained ACTH immunoactive material with apparent MW of 36,000, 24,000 and 4500 daltons. The highest MW form of ACTH co-migrated with a peak of endorphin immunoactive material. No material the size of glycosylated ACTH(1-39) was detected. Separated forms of high MW ACTH prepared from mouse tumor cell culture medium stimulated the same maximal production of steroid as ACTH(1-39) in the guinea pig adrenal cell bioassay. Pro-ACTH/endorphin and ACTH biosynthetic intermediate were 2 orders of magnitude less potent than synthetic human ACTH(1-39); glycosylated ACTH(1-39) was equipotent to ACTH(1-39) although no similar material was detected in guinea pig pituitary extracts. Isolated guinea pig adrenal cortical cells were incubated with the various separated forms of mouse tumor cell ACTH and products synthesized from (3H)pregnenolone were analyzed by 2-dimensional TLC. The ratio of cortisol-related to corticosterone-related products was the same in response in glycosylated and nonglycosylated ACTH. [Synthetic camet .beta.-endorphin was used as the standard in all endorphin immunoassays.].

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005481842

Download citation: RISBibTeXText

Related references

Biological activity of high molecular weight forms of acth. Federation Proceedings 37(6): 1823, 1978

Forms and activity of high molecular weight adrenocorticotropin in guinea pig. Peptides 1(3): 223-229, 1980

High molecular weight forms of immunoreactive ACTH in a human pituitary and ectopic ACTH-producing tumors. Endocrinologia Japonica 27(4): 405-410, 1980

Physiological effects of high molecular weight forms of acth. Journal of Supramolecular Structure 9(Suppl. 4): 173, 1980

High molecular weight forms of human ACTH are glycoproteins. Journal of Clinical Endocrinology and Metabolism 44(1): 214-217, 1977

High molecular weights forms of ACTH in a commercial Argentinian preparation. Acta Physiologica Latino Americana 26(2): 154-155, 1976

In vitro effects of high molecular weight forms of ACTH on the fetal sheep adrenal. Nature 284(5757): 616-618, 1980

Comparison of biological activity of high molecular weight and low molecular weight forms of immunoreactive prolactin from human serum in cultured lymphoma NB2 cells. Voprosy Meditsinskoi Khimii 42(3): 234-239, 1996

Prostaglandin E2 administered to fetal sheep increases the plasma concentration of adrenocorticotropin (ACTH) and the proportion of ACTH in low molecular weight forms. Endocrinology 136(3): 1233-1240, 1995

Differential Modulation of Cell Phenotype by Different Molecular Weight Forms of Basic Fibroblast Growth Factor: Possible Intracellular Signaling by the High Molecular Weight Forms. Journal of Cell Biology 129(1): 233-243, 1995

High molecular weight interfering activity in acth radio immunoassay. Endocrine Society of Australia Proceedings 24: 53, 1981

High molecular weight forms of aminoacyl-tRNA synthetases in animals. I. Amount and enzymatic composition of high molecular weight synthetase in cytosol from rabbit liver. Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina 48: 29-34, 1993

Steroidogenic activity of high molecular weight forms of corticotropin. Biochemistry 18(19): 4215-4224, 1979

High molecular weight forms of egf like activity in urine from patients with cervical cancer. Journal of Cancer Research and Clinical Oncology 116(Suppl. Part 1): 134, 1990

The interrelations between high molecular weight and low molecular weight forms of normal and mutant krabbe disease galacto cerebrosidase ec 3.2.1.46. Biochemical Journal 189(1): 9-16, 1980