Home
  >  
Section 6
  >  
Chapter 5,499

Further studies on the quantitation of the hemo globin a hemo globin s hemo globin c and hemo globin f in new born babies with different hemo globinopathies using high pressure liquid chromatography

Gardiner, M.B.; Carver, J.; Abraham, B.L.; Wilson, J.B.; Huisman, T.H.J.

Hemoglobin 6(1): 1-14

1982

ISSN/ISBN: 0363-0269
Accession: 005498928
Download citation:  
Text
  |  
BibTeX
  |  
RIS
High pressure liquid chromatography (HPLC) was used for the detection and quantitation of the .beta. chain variants Hb S and Hb C in blood samples of newborn babies with different hemoglobinopathies. The complete separation of the Hb C, S, A and F made it possible to diagnose conditions such as AS, AC, SS, CC, SC and even S(C)-.beta.+ thalassemia. The procedure was fast (62 min) and ideally suited for the quantitation of Hb F at birth. Data for a few hundred cord blood samples indicated a great variability in the relative quantities of Hb S or Hb C in heterozygotes which prevents a definitive diagnosis of a simultaneously occurring .alpha.-thalassemia except perhaps of the homozygous form of .alpha.-thalassemia-2 (.alpha.o.alpha./.alpha.o.alpha.). Some overlap between the quantitative results in Hb S (or Hb C) heterozygotes and in babies with the Hb S (Hb C)-.beta.-thalassemia condition was observed.

PDF emailed within 1 workday: $29.90




Related References

Charlesworth, D. 1975: A new hemo globin variant hemo globin f sardinia gamma 75 e 19 iso leucine replaced by threonine found in a family with hemo globin g philadelphia beta chain deficiency and a lepore like hemo globin indistinguishable from hemo globin a 2 Acta Haematologica (basel): 347-355
Abraham, E.C.; Huisman, T.H.J.; Schroeder, W.A.; Pace, L.A.; Grussing, L. 1977: Micro chromatography of hemo globins part 7 detection of some uncommon hemo globin variants and 2 rapid methods for the quantitation of hemo globin a 2 in the presence of hemo globin c Journal of Chromatography 143(1): 57-63
Adachi, K.; Asakura, T. 1981: Gelation and crystallization of sickle hemo globin s and hemo globin charlem and nonsickle hemo globin hemo globin a and hemo globin c in concentrated phosphate buffer Brewer, G J (Ed ) Progress in Clinical And Biological Research, Vol 55 The Red Cell; 5th International Conference, Ann Arbor, Mich , Usa, Sept 29-Oct 2, 1980 Xxxiii+806p Alan R Liss, Inc : New York, N Y , Usa Illus P123-142
Siggaard Andersen, O.; Norgaard Pedersen, B.; Rem, J. 1972: Hemo globin pigments spectrophotometric determination of oxy hemo globin carboxy hemo globin met hemo globin and sulf hemo globin in capillary blood Clinica Chimica Acta 42(1): 85-100
Brnjas Kraljevic, J.; Maricic, S.; Pifat, G. 1976: Hydration of oxy hemo globin carboxy hemo globin deoxy hemo globin and met hemo globin a proton magnetic relaxation study of protein self association in concentrated solutions Periodicum Biologorum 78(2): 158
Sumida, I. 1975: Studies of abnormal hemo globins in western japan frequency of visible hemo globin variants and chemical characterization of hemo globin sawara alpha 2 6 alanine beta 2 and hemo globin mugino hemo globin ferrara alpha 2 4 glycine beta 2 Japanese Journal of Human Genetics 19(4): 343-364
Sinet, M.; Muffat Joly, M.; Pocidalo, J.J. 1977: Hemo globin oxygen affinity dependence of hemo globin and 2 3 di phospho glycerate concentration in the erythrocyte comparison between hemo globin a and hemo globin s INSERM (Institut National de la Sante et de la Recherche Medicale) Colloque 70: 33-40
Lambert, W.J. 1980: An instrument for the measurement of total hemo globin oxy hemo globin carboxy hemo globin and met hemo globin in the blood of dogs rats and other animals Clinical Chemistry: 1046
Apell, G. 1972: Chemical heterogeneity of fetal hemo globin in subjects with sickle cell anemia homozygous hemo globin c disease hemo globin sc disease and various combinations of hemo globin variants Clinica Chimica Acta: 5-16
Henson, J.; Huisman, T.H.J. 1978: Possible relationship between the level of hemo globin barts gamma 4 and the relative amount of hemo globin s or hemo globin c in black hetero zygous new born Hemoglobin 2(4): 391-398
Juckett, D.A.; Hultquist, D.E. 1984: Magnetic circular dichroism studies of hemo globin the reduction of ferri hemo globin by ferro cytochrome beta 5 and characterization of the high spin hydroxy species of mixed valence hemo globin Biophysical Chemistry 19(4): 321-336
Boissel, J.P.; Fabritius, H.; Richard, P.; Wajcman, H.; Cabannes, R.; Labie, D. 1981: Polymorphism of hemo globins d in ivory coast hemo globin korle bu hemo globin avicenna and hemo globin cocody Nouvelle Revue Francaise d'Hematologie 23(4): 197-202
Bektas, M.S.; Bieber, F.A.; Braunitzer, G. 1981: Hemo globins 41. the embryonic hemo globins of mammals a new hemo globin hemo globin heide ii and demonstration of the hemo globin structure of gower i in the pig embryo Hoppe Seyler's Zeitschrift fuer Physiologische Chemie 362(7): 991-996
Garrick, L.M.; Mcdonald, M.J.; Shapiro, R.; Bleichman, M.; Mcmanus, M.; Bunn, H.F. 1980: Structural analysis of the minor human hemo globin components hemo globin a i a 1 hemo globin a i a 2 and hemo globin a i b European Journal of Biochemistry 106(2): 353-360
Charache, S. 1971: Physical studies of human adult hemo globin a and hemo globin chesapeake and their implication to the nature of oxygenation of hemo globin Arends, Temski And R.l. Nagel (ed.). Genetical, Functional, And Physical Studies Of Hemoglobins. Symposium. Viii 293p. Illus. Map. S. Karger: Basel, Switzerland; New York, N.y., U.s.a. 238-248
Rosenberg, M.R. 1968: In vivo and in vitro interactions of human hemo globins a hemo globin s and hemo globin c with a variant hemo globin e Nature (London) 219(5158): 1042-1044
Efremov, G.D.; Huisman, T.H.J. 1974: Studies on the heterogeneity of hemo globin part 15 separation of fetal hemo globin and the normally occurring minor adult hemo globins by chromatography on deae cellulose Journal of Chromatography 89(2): 191-196
Baig, M.A.; Ansari, A.A.; Malling, H.V. 1980: Development of in vivo somatic mutation system using hemo globin variants of mouse preparation and use of anti s hemo globin antibody in the detection of red cells containing d hemo globin Environmental Mutagenesis 2(2): 296
Dijkhuizen, P.; Buursma, A.; Gerding, A.M.; Zijlstra, W.G. 1977: Sulf hemo globin absorption spectrum millimolar extinction coefficient at 620 nanometers and interference with the determination of hemo globin and of hemo globin cyanide Clinica Chimica Acta 78(3): 479-488
Fesus, L. 1980: Less hemo globin aa and hemo globin bb type offspring and poor lambing results of hemo globin aa ewes in a herd of hungarian merino sheep Animal Blood Groups and Biochemical Genetics 11(Suppl 1): 23