Section 6
Chapter 5,499

Further studies on the quantitation of the hemo globin a hemo globin s hemo globin c and hemo globin f in new born babies with different hemo globinopathies using high pressure liquid chromatography

Gardiner, M.B.; Carver, J.; Abraham, B.L.; Wilson, J.B.; Huisman, T.H.J.

Hemoglobin 6(1): 1-14


ISSN/ISBN: 0363-0269
Accession: 005498928

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High pressure liquid chromatography (HPLC) was used for the detection and quantitation of the .beta. chain variants Hb S and Hb C in blood samples of newborn babies with different hemoglobinopathies. The complete separation of the Hb C, S, A and F made it possible to diagnose conditions such as AS, AC, SS, CC, SC and even S(C)-.beta.+ thalassemia. The procedure was fast (62 min) and ideally suited for the quantitation of Hb F at birth. Data for a few hundred cord blood samples indicated a great variability in the relative quantities of Hb S or Hb C in heterozygotes which prevents a definitive diagnosis of a simultaneously occurring .alpha.-thalassemia except perhaps of the homozygous form of .alpha.-thalassemia-2 (.alpha.o.alpha./.alpha.o.alpha.). Some overlap between the quantitative results in Hb S (or Hb C) heterozygotes and in babies with the Hb S (Hb C)-.beta.-thalassemia condition was observed.

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