+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gallium 67 binding to sulfated proteoglycan in normal and carbon tetrachloride damaged liver



Gallium 67 binding to sulfated proteoglycan in normal and carbon tetrachloride damaged liver



Nuclear Medicine & Biology 13(5): 573-576



The binding of 67Ga and 59Fe to sulfated proteoglycan in the normal and CCl4-damaged mouse livers have been studied by means of Sepharose CL-4B column chromatograpy in guanidine hydrochloride. 67Ga uptake in the liver was elevated to approximately 1.8 times that of the control 2 days after a single adminstration of CCl4. 67Ga was bound to two types of sulfated proteoglycan (average mol. wt 1,700,000 and 35,000) in the normal and CCl4-damaged liver. However, the percentage of 67Ga bound to sulfated proteoglycan (mol. wt about 1,700,000) in the CCl4-damaged liver was increased as compared with that in the normal liver. Papain digestion of these sulfated protelygan fractions caused the 67Ga radioactivities to shift to a low moelcular weight fraction (about 10,000). Cellulose acetate electrophoresis of this fraction (mol. wt about 10,000) isolated from the normal and CCl4-damaged livers resulted in identifying heparan sulfate(HS). The 35SO4 incorporation into HS band isolated from the CCl4-damaged liver, as an indicator of HS synthesis, was increased compared with that of the normal liver. 59Fe was not bound to sulfated proteoglycan and its gel filtration pattern was evidently different from that of 67Ga.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005500730

Download citation: RISBibTeXText


Related references

67Ga binding to sulfated proteoglycan in normal and CCl4-damaged liver. International Journal of Radiation Applications and Instrumentation. Part B Nuclear Medicine and Biology 13(5): 573-576, 1986

In vivo and in vitro nidogen liver gene expression studies in carbon tetrachloride damaged rat liver and in normal liver cells. Journal of Hepatology 11(Suppl. 2): S52, 1990

The metabolism of damaged tissue, XVII. Turnover rate of ATP, glucose-6-phosphate and fructose-6-phosphate in the liver of normal and carbon-tetrachloride damaged mice. Zeit Physiol Chem 332(1/6): 204-215, 1963

Propranolol levels in serum heart and liver in normal and carbon tetrachloride liver damaged rats under coadministration of lidocaine. Naunyn-Schmiedeberg's Archives of Pharmacology 330(Suppl.): R12, 1985

Studies on glycogen metabolism in the liver of normal and carbon tetrachloride-damaged rats. Nuclear-Medizin 1: 182-193, 1959

Investigations on the effect of the portal vein obstruction in the normal and carbon-tetrachloride-damaged rat liver. Z Gesamte Exp Med 141(1): 1-16, 1966

Concentration of C14 labeled carbon tetrachloride in normal and previously damaged mouse liver. In: Annual meeting of the American Association for the Study of Liver Diseases, 1962. Gastroenterology 44(4): 485, 1963

Ultracytochemical localization of adenylate cyclase in liver of normal and of carbon tetrachloride and carbon tetrachloride plus colchicine treated rats. Cellular & Molecular Biology 34(6): 621-628, 1988

Studies on the antihepatotoxic effect of a liver extract as illustrated in the model of a rat liver damaged by carbon tetrachloride. Die Medizinische Welt 25(4): 150-152, 1974

The Effect of Raw Liver Extract on the Carbon Tetrachloride-Damaged Rat Liver. Acta Hepato-Splenologica 11: 27-34, 1964

Changes in transglutaminase activity in carbon tetrachloride-damaged rat liver. Experientia 47(1): 70-75, 1991

Histochemical investigations on the carbon tetrachloride damaged rabbit liver. Acta Biologica et Medica Germanica 2(1): 7, 1959

Uptake of gallium-67 by the hepatocytes during liver regeneration in carbon tetrachloride-treated mice. Biological & Pharmaceutical Bulletin 27(12): 1913-1915, 2004

Hepatocyte heterogeneity in dbsp transport in normal and carbon tetrachloride damaged livers. Hepatology 8(5): 1366, 1988

Influence of bovine somatotropin on the liver experimentally damaged with carbon tetrachloride. Archivum Immunologiae et Therapiae Experimentalis 23(4): 537-541, 1975