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General method for the purification of lipids for surface pressure studies application to mono galactosyl di glyceride

General method for the purification of lipids for surface pressure studies application to mono galactosyl di glyceride

Journal of Chromatography 207(3): 387-394

To prepare lipids free of surface-active contaminants and suitable for the measurement of surface pressure isotherms, a general purification method was developed involving preparative TLC. A detailed description of the procedure and blank experiments carried out with a monolayer trough are presented. The method provides a rapid purification of various classes of lipids, the whole procedure taking less than 1 day. It gives highly reproducible results, better than 2 .ANG.2 per molecule at any surface pressure. As an example of an application, the results obtained with a commercial sample of [spinach leaf] monogalactosyldiglyceride, a major plant lipid, are reported.

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Accession: 005508783

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