Generation of memory cells part 3 antibody class requirements for the generation of bone marrow derived memory cells by antigen antibody complexes
Immunology 37(2): 345-352
Immunizing mice with preformed antigen-antibody complexes is a highly effective means of generating B [bone marrow-derived]-memory cells. The capacity of mouse anti-dinitrophenyl(DNP) Ig[immunoglobulin]M, IgG1, IgG2 and IgA antibodies were compared to generate DNP-specific memory, when given in complex with antigen (DNP-KLH; keyhole limpet hemocyanin). Monoclonal IgM antibodies (2) exerted no adjuvant effect, whereas a monoclonal IgA antibody was effective. IgG2 antibodies were a more powerful adjuvant than IgG1, regardless of whether anti-DNP or anti-KLH antibodies were used. The capacity of DNP-KLH-antibody complexes to localize in splenic lymphoid follicles were ranked IgG2 > IgG1 > IgA; IgM complexes did not localize in follicles. The results correlate with data (presented elsewhere) on the capacity of the different antibodies to activate mouse complement, and confirm that C [complement] activation is an essential requirement for both follicular localization of immune complexes and for the generation of B memory cells. Although activation of the alternative complement pathway is sufficient to effect both processes, the results with IgG2 antibodies raise the possibility that classical pathway activation may be more effective.