+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation

Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation

Journal of Bacteriology 170(1): 141-148

When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet Western blots revealed that some mutants still produced MDH protein. Complementation of 50 mutants by a cosmid gene bank of M. organophilum XX demonstrated that three major regions of the genome, each of which was separated by a minimum of 40 kilobases, were required for expression of active MDH. By subcloning and Tn5 insertion mutagenesis of subcloned fragments, at least 11 genes clustered within these three regions were subsequently identified. The identity of the MDH structural gene, which was initially determined by hybridization to the structural gene of Methylobacterium sp. strain AM1, was confirmed by Western blot analysis of an MDH-.beta.-galactosidase fusion protein.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005512248

Download citation: RISBibTeXText

PMID: 2826390

DOI: 10.1128/jb.170.1.141-148.1988

Related references

Cloning mapping and nucleotide sequence of a mouse erythrocyte beta spectrin complementary dna. Cell Differentiation 20(Suppl.): 47S, 1987

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance. Clinical Microbiology Reviews 1(2): 228-243, 1988

Construction of bonded ceramic occlusal surface. Zahntechnik 33(1): 38-42, 1975

Nucleotide sequence of the murine leukaemia virus amphotropic strain 4070A integrase (IN) coding region and comparative structural analysis of the inferred polypeptide. Archives of Virology 142(9): 1757-1770, 1997

Cloning and genetic analyses of two highly polymorphic moderately repetitive nuclear dnas from phytophthora infestans. Current Genetics. 22(2): 107-115, 1992

1,3--Glucanase, 1,6--Giucanase and -Glucosidase Activities of Sclerotium glucanicum: Synthesis and Properties. Microbiology 135(11): 2847-2858, 1989

New expression of myelomonocytic antigens by microglia and perivascular cells following lethal motor neuron injury. Journal of Neuroimmunology 27(2-3): 121-132, 1990

Regulation of metastatic ovarian cells in serum free medium by epidermal growth factor and transferrin. Journal of Cellular Physiology 104(2): 233-240, 1980

Regulation of metallothionein gene expression. Progress in nucleic acid research and molecular biology6(66): 357-384, 2001

Identification of metaplastic variants generated by transfection of a nonmetastatic rat mammary epithelial cell line with DNA from a metastatic rat mammary cell line. Americal Journal of Pathology 137(3): 629-641, 1990

Nucleotide sequence and transcriptional start point of the kan gene encoding an aminoglycoside 3 n acetyltransferase from streptomyces griseus ss 1198pr. Gene (Amsterdam) 108(1): 127-132, 1991

Optimization of growth conditions for the production of proteolytically sensitive proteins in the periplasmic space of escherichia coli. Applied Microbiology and Biotechnology 36(1): 14-20, 1991

The methanol hemisolvate of amiloride hydrochloride. Acta Crystallographica. Section C Crystal Structure Communications 57(Pt 10): 1217-1219, 2001

Genetic transformation in local strains of moraxella osloensis. Zhonghua Weishengwuxue He Mianyixue Zazhi 7(5): 293-295, 1987

Promoters and transcripts associated with the aroP gene of Escherichia coli. Journal Of Bacteriology. 179(13): 4206-4212, 1997