Gibberellic acid stimulated and auxin stimulated cell growth in relation to rna synthesis protein synthesis and development in the wheat triticum aestivum cultivar gamenya coleoptile

Rose, R.J.; Crossman, J.B.

Physiologia Plantarum 55(3): 395-401

1982


ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1982.tb00311.x
Accession: 005524020

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Young excised coleoptiles from dark grown wheat have their cell growth promoted by GA3, while sections from older coleoptiles have their cell growth promoted by auxin. The GA3 response has a much longer lag period than that of auxin. Neither GA3 nor auxin has any effect on 14C-leucine and 14C-uridine incorporation and uptake after 1 h, indicating that the lag in growth stimulation following GA3 application is not associated with changes in protein or RNA synthesis. Following a 6 h incubation there are small increases in 14C-leucine and 14C-uridine incorporation in response to GA3 and auxin and in the case of auxin, this is associated with increased uptake. Studies on protein and RNA turnover using pulse-chase experiments showed that GA3 and auxin have no effect on protein and RNA stability. There are developmental changes in RNA and protein synthesis that should be considered in any explanation of the mechanism of action of these hormones on cell growth. Young GA3-sensitive tissue has high rates of RNA synthesis and low protein and RNA turnover, while auxin-sensitive tissue has low rates of RNA synthesis, slightly higher rates of RNA turnover and much higher rates of protein turnover. More effective utilization by GA3 and auxin of a basal control level of RNA and protein synthesis and turnover in coleoptile tissue is evidenced.

Gibberellic acid stimulated and auxin stimulated cell growth in relation to rna synthesis protein synthesis and development in the wheat triticum aestivum cultivar gamenya coleoptile