Glycine fermentation via a glycine reductase in peptococcus glycinophilus and peptococcus magnus

Duerre, P.; Spahr, R.; Andreesen, J.R.

Archives of Microbiology 134(2): 127-135

1983


ISSN/ISBN: 0302-8933
DOI: 10.1007/bf00407945
Accession: 005530032

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Abstract
P. glycinophilus and P. magnus (P. variabilis) utilized only glycine-containing compounds for growth and required Se compounds for the fermentation of glycine in an optimized medium. Under these conditions an active glycine reductase was expressed in vivo as demonstrated for both species by tracer experiments and additionally in vitro for P. glycinophilus in cell extracts. The availability of Se determines the fermentation pathway of glycine. In the presence of selenite, glycine is converted via the glycine reductase rather than the previously known glycine-serine-pyruvate interconversion. The molar growth yield of P. magnus was determined to be 8.9 g of dry wt/mol of glycine. The significance of the low vitamin B12 content and a low carbon monoxide dehydrogenase activity in P. glycinophilus for the reduction of CO2 to acetate is discussed.

Glycine fermentation via a glycine reductase in peptococcus glycinophilus and peptococcus magnus