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Golgi apparatus secretion in plasmolyzed pisum sativum



Golgi apparatus secretion in plasmolyzed pisum sativum



Protoplasma 90(3-4): 369-379



Stem segments of P. sativum L. when plasmolyzed with mannitol show a continued secretion through the dictyosome-vesicle pathway. While incorporation into the cell wall is inhibited during plasmolysis, the secreted material, expressed as an ethanol insoluble fraction of the homogenate supernatant after removal of cell organelles and cell wall, apparently accumulates between the protoplast and cell wall. The inhibition of cell wall incorporation and the accumulation of ethanol insoluble material may be reversed on removal of the mannitol. The relevance of this effect for nonplasmolyzing turgor changes is discussed.

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