Grain yield and yield components of regenerated wheat triticum aestivum plants in sc 4 generation
Eapen, S.; Suseelan, K.N.; Bhagwat, S.G.; Rao, P.S.; Bhatia, C.R.
Proceedings of the Indian National Science Academy Part B Biological Sciences 51(5): 627-632
Plants were regenerated from callus cultures initiated from mature embryo explants of two bread cultivars Kalyan Sona and NI-4. The regenerated (SC-1) plants of cultivar NI-4 showed chlorophyll variants such as albino and striata. No such variant was observed in cv. Kalyan Sona. Progenies of NI-4 segregated for chlorophyll variants up to SC-3 generation. The highest yielding SC-3 plants derived from each of the original SC-1 plants were selected and their progenies evaluated for grain yield and yield components in replicated field experiments. All the 18 regenerated lines of Kalyan Sona were statistically similar to the control in grain yield. Most of the yield components, gliadins and high molecular weight glutenins were comparable to the parent. In cultivar NI-4, which showed greater phenotypic variability, 6 out of 12 regenerated lines were equal in grain yield. The results indicate that some of the unwanted genetic alterations induced by in vitro culture are partly eliminated by subsequent cycles of sexual reproduction and can be further reduced by conventional plant breeding methods of selecting parental and higher yielding single plants to recover lines equal in productivity to the original cultivar.