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Growth and coloration of tradescantia stamen hairs cultured in vitro



Growth and coloration of tradescantia stamen hairs cultured in vitro



Environmental & Experimental Botany 22(2): 255-262



Young stamens of Tradescantia clone 02 were cultured in vitro on agar media containing various combinations of 3 plant hormones, IAA, GA3 and kinetin. Maximum growth of stamen hairs occurred with medium containing 5.0 mg/l IAA, 1.0 mg/l GA3 and 0.1 mg/l kinetin. As much as a 10 cell increase occurred in stamen hairs on this medium, but the growth did not equal the normal in vivo growth. The hormonal concentrations of the medium which induced maximum coloration were 0.1 mg/l IAA, 1.0 mg/l GA3 and 0.1 mg/l kinetin. Higher concentrations of sucrose also seemed effective in promoting coloration. The distribution pattern of colored cells along hairs (more coloration at hair tips) supports the idea that completion of cell division is one of the triggers of coloration. The possibility of developing in vitro culture techniques of Tradescantia stamens is suggested. [Tradescantia is a valuable tool in the detection of environmental mutagens.].

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Accession: 005537600

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DOI: 10.1016/0098-8472(82)90047-8



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