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Growth auxin requirements and iaa content of cultured crown gall and habituated tissues of tobacco nicotiana tabacum cultivar havana 425



Growth auxin requirements and iaa content of cultured crown gall and habituated tissues of tobacco nicotiana tabacum cultivar havana 425



Differentiation 25(2): 101-105



Radioimmunoassay was used to compare the IAA concentration in cultured auxinautonomous tissues of N. tabacum L. cv. Havana 425 that differ in growth rate, auxin requirement, capacity for organogenesis and proximal cause of transformation. Lines HT37 and HB6 were crown-gall tissues transformed by the T37 and B6 strains of Agrobacterium tumefaciens. The tissue line 156AH, like the crown gall lines, was auxin and cytokinin autotrophic, but arose spontaneously in culture and did not result from crown gall transformation. The IAA content of the lines was variable and between 10-7 and 10-6 mol kg-1 fresh weight. This same range of IAA concentration was found in growing leaf tissues on the plant. IAA concentrations were 2-5 fold higher in HB6 than HT37 during the first few days of culture, and exogenous auxin induced the teratomatous HT37 tissue to grow in an unorganized fashion suggesting that auxin plays a role in regulating tumor morphology. The major difference between genetically transformed crown gall tissues and the auxin-cytokinin autotrophic tissue line was that net IAA production continued to rise for several weeks of culture in tumor tissues but only for the first few days of culture in the autotrophic tissue.

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