EurekaMag
+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

Growth hormone secretion from chicken adenohypophyseal cells in primary culture: effects of human pancreatic growth hormone-releasing factor, thyrotropin-releasing hormone, and somatostatin on growth hormone release


Growth hormone secretion from chicken adenohypophyseal cells in primary culture: effects of human pancreatic growth hormone-releasing factor, thyrotropin-releasing hormone, and somatostatin on growth hormone release



General and Comparative Endocrinology 65(3): 408-414



ISSN/ISBN: 0016-6480

PMID: 2881841

DOI: 10.1016/0016-6480(87)90126-2

A primary culture of chicken adenohypophyseal cells has been developed to study the regulation of growth hormone (GH) secretion. Following collagenase dispersion, cells were exposed for 2 hr to vehicle (control) or test agents. Human pancreatic (tumor) growth hormone-releasing factor (hpGRF) and rat hypothalamic growth hormone-releasing factor stimulated GH release to similar levels. GH release was increased by the presence of dibutyryl cyclic AMP. Thyrotropin-releasing hormone (TRH) alone did not influence GH release: however, TRH plus hpGRF together exerted a synergistic (greater than additive) effect, increasing GH release by 100 to 300% over the sum of the values for each secretagogue acting alone. These relationships between TRH and hpGRF were further examined in cultured cells exposed to secretagogues for two consecutive 2-hr incubations. TRH pretreatment enhanced subsequent hpGRF-stimulated GH release by about 80% over that obtained if no secretagogue was present during the first incubation. In other experiments, somatostatin (SRIF) alone did not alter GH secretion. However, SRIF reduced hpGRF-stimulated GH release to levels found in controls. Furthermore, GH release stimulated by the presence of both TRH and hpGRF was lowered to control values by SRIF. The results of these studies demonstrate that a primary culture of chicken adenohypophyseal cells is a useful model for the study of GH secretion. Indeed, these results suggest that TRH and hpGRF regulate GH secretion by mechanisms which are not identical.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 005540331

Download citation: RISBibTeXText

Related references

Possible participation of calcium in growth hormone release and in thyrotropin-releasing hormone and human pancreatic growth hormone-releasing factor synergy in a primary culture of chicken pituitary cells. General and Comparative Endocrinology 75(3): 481-491, 1989

Interaction of human pancreatic growth hormone-releasing factor, thyrotropin-releasing hormone, and somatostatin on growth hormone release in chickens. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 183(3): 363-367, 1986

Thyrotropin-Releasing Hormone (TRH) and Somatostatin (SST), but not Growth Hormone-Releasing Hormone (GHRH) nor Ghrelin (GHRL), Regulate Expression and Release of Immune Growth Hormone (GH) from Chicken Bursal B-Lymphocyte Cultures. International Journal of Molecular Sciences 21(4), 2020

Possible participation of calcium in growth hormone release and in trh and human pancreatic growth hormone releasing factor synergy in a primary culture of chicken pituitary cells. General and Comparative Endocrinology 76(3): 481-491, 1989

Interactions of trh human pancreatic growth hormone releasing factor prostaglandin e 1 prostaglandin e 2 and prostaglandin f 2 alpha on growth hormone release from a culture of chicken adenohypophyseal cells. Federation Proceedings 44(3): 631, 1985

Effect of thyrotropin-releasing hormone on growth hormone release in normal subjects pretreated with human pancreatic growth hormone-releasing factor 1-44 pulsatile administration. Neuroendocrinology 44(4): 470-474, 1986

Growth hormone release is stimulated by thyrotropin releasing hormone and human pancreatic growth hormone releasing factor in control sex linked and autosomal dwarf white leghorn chickens. Poultry Science 63(Suppl 1): 35-36, 1984

Interaction between human pancreatic growth hormone releasing factor (hpGRF) and thyrotropin releasing hormone (TRH) on growth hormone secretion in domestic fowl. Hormone and Metabolic Research 17(2): 113-114, 1985

Spontaneous daily fluctuations and the effect of thyrotropin releasing hormone melanocyte stimulating hormone release inhibiting hormone and luteinizing hormone releasing hormone on growth hormone secretion during florid acromegaly. Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin 81: 1521-1523, 1975

Somatostatin inhibition of thyrotropin-releasing hormone- and growth hormone-releasing factor-induced growth hormone secretion in young and adult anesthetized chickens. General and Comparative Endocrinology 75(2): 256-264, 1989

Potent interaction between trh and human pancreatic growth hormone releasing factor in stimulating chicken growth hormone in vivo hypothalamic noradrenergic mediation in trh stimulation of chicken growth hormone release. Domestic Animal Endocrinology 2(4): 183-190, 1985

Growth hormone/insulin-like growth factor-1 response to acute and chronic growth hormone-releasing peptide-2, growth hormone-releasing hormone 1-44NH2 and in combination in older men and women with decreased growth hormone secretion. Endocrine 14(1): 79-86, 2001

Studies on the response of growth hormone (GH) secretion to GH-releasing hormone, thyrotropin-releasing hormone, gonadotropin-releasing hormone, and somatostatin in acromegaly. Journal of Clinical Endocrinology and Metabolism 63(1): 167-173, 1986

Studies on the responses of growth hormone (GH) secretion to GH-releasing hormone, thyrotropin-releasing hormone, gonadotropin-releasing hormone, and somatostatin in acromegaly. The Journal of Clinical Endocrinology and Metabolism 63(1): 167-173, 1986

The interrelationship between the effects of somatostatin and human pancreatic growth hormone-releasing factor on growth hormone release by cultured pituitary tumor cells from patients with acromegaly. Journal of Clinical Endocrinology and Metabolism 58(2): 250-254, 1984