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H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line



H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line



Immunogenetics 4(2): 105-115



A single-step selection procedure, using anti-H-2 antibodies in the presence of complement, is described for the isolation of H-2 variants from a cultured heterozygous mouse Friend leukemia [b/d] cell line. The variants obtained are hemizygous, i.e., they express only 1 of the 2 parental sets of H-2 antigens present in the genome of the wild-type cells. The phenotypes of the variants are stable, and no revertants were detected after more than 200 generations in culture. The variants retain 2 copies of chromosome 17, as do the wild-type cells. Chromosome loss does not appear to be the mechanism for the emergence of these variants.

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Accession: 005545265

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