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H 2 antigen variants in a cultured mouse leukemia cell line part 2 effect of selection against a k end gene



H 2 antigen variants in a cultured mouse leukemia cell line part 2 effect of selection against a k end gene



Immunogenetics 5(4): 295-308



The effect of immune selection against a single gene product was investigated in a cultured mouse Friend leukemia cell line [HFL-b/d.4.4.1]. The clonal cell line used is heterozygous at the H-2 complex and expresses the H-2d and H-2b haplotypes. The genes selected against were the H-2K locus alleles. Variants were obtained after a single-step selection using anti-H-2Kb or anti-H-2Kd serum. The phenotypes of the variants obtained showed an interesting asymmetry between the 2 haplotypes. Selection against the H-2Kb allele resulted in the isolation of the 2 expected types of variants-those that had lost only H-2Kb and those that had lost H-2Kb and the linked H-2Db. Selection against H-2Kd yielded, exclusively, variants that lost the selected antigen and the linked H-2Dd. None of the variants showed an alteration in expression of antigens in trans configuration. Karyotypic analyses of the variants revealed that all the cells had retained both copies of chromosome 17 present in the wild-type cells. The variants probably did not emerge through chromosome loss.

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Accession: 005545271

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