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Hetero duplex analysis of molecular clones of the pathogenic friend virus complex friend murine leukemia virus friend mink cell focus forming virus and the polycythemia inducing and anemia inducing strains of friend spleen focus forming virus






Journal of Virology 51(2): 306-314

Hetero duplex analysis of molecular clones of the pathogenic friend virus complex friend murine leukemia virus friend mink cell focus forming virus and the polycythemia inducing and anemia inducing strains of friend spleen focus forming virus

Heteroduplex mapping of molecular clones of the Friend virus complex, which includes the replication-competent ecotropic Friend murine leukemia virus (F-MuLV) cell focus-forming virus (F-MCF), and replication-defective polycythemia- and anemia-inducing strains of spleen focus-forming virus (SFFVp and SFFVa, respectively), was employed to provide insight into the molecular basis of their relationships. In heteroduplexes of F-MuLV .times. F-MCF, a major substitution of 0.89 kilobases in the env gene of F-MCF was discerned. Heteroduplexes of SFFVp .times. F-MuLV or F-MCF, and SFFVa .times. F-MuLV or F-MCF showed several major deletions in the pol gene region and a single major deletion in the 3' half of the env gene region of SFFVp and SFFVa. A major substitution of 0.89 kilobases was mapped to the 5' end of the env deletion of SFFVp and SFFVa in heteroduplexes with F-MuLV, similar to that seen in F-MuLV .times. F-MCF heteroduplexes. This env gene region was totally homologous in F-MCF .times. SFFVp or SFFVa and SFFVp .times. SFFVa heteroduplexes. Thus, SFFVp and SFFVa lack part of the env gene at its 3' end, corresponding to the p15(E) coding region; major deletions occur in the pol and env genes which account for the replication defectiveness of SFFVp and SFFVa; minor substitutions occur in the gag gene region of SFFVa that are not present in SFFVp, F-MuLV or F-MCF; a major substitution exists in the gp70 region of the env gene between F-MuLV and F-MCF that probably accounts for the differences in their host range specificities; this substitution in F-MCF is identical to the gp70 part of the gp52 coding region of SFFVp and SFFVa; and heteroduplexes of F-MCF show unambiguously that no additional large substitutions are present in SFFVp or SFFVa that could account for differences in their leukemogenicity.

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Accession: 005564946



Related references

Heteroduplex analysis of molecular clones of the pathogenic Friend virus complex: Friend murine leukemia virus, Friend mink cell focus-forming virus, and the polycythemia- and anemia-inducing strains of Friend spleen focus-forming virus. Journal of Virology 51(2): 306-314, 1984

Multipotential hemopoietic cell lines isolated from stem cell cultures infected with friend virus complex murine leukemia virus plus friend spleen focus forming virus show presence of murine leukemia virus but not friend spleen focus forming virus. Leukemia Research 10(2): 187-194, 1986

New cell surface gp 70 related to friend mink cell focus inducing virus is expressed on friend virus induced erythro leukemic spleen cells after elimination of ecotropic friend virus gp 70 in rfv 3r s mice. Journal of Experimental Medicine 154(3): 868-882, 1981

Helper-independent mink cell focus-inducing strains of Friend murine type-C virus: potential relationship to the origin of replication- defective spleen focus-forming virus. Journal of Experimental Medicine 148(3): 639-653, 1978

Helper-independent mink cell focus-inducing strains of Friend murine type-C virus: potential relationship to the origin of replication-defective spleen focus-forming virus. Journal of Experimental Medicine 148(3): 639-653, 1978

Sequence comparisons of the anemia inducing and polycythemia inducing strains of friend spleen focus forming virus. Journal of Virology 53(2): 570-578, 1985

Conversion of Friend mink cell focus-forming virus to Friend spleen focus-forming virus by modification of the 3' half of the env gene. Journal of Virology 65(1): 132-137, 1991

Sequence comparisons of the anemia- and polycythemia-inducing strains of Friend spleen focus-forming virus. Journal of Virology 53(2): 570-578, 1985

Leukemia induction by a new strain of Friend mink cell focus-inducing virus: synergistic effect of Friend ecotropic murine leukemia virus. Journal of Virology 51(1): 63-70, 1984

Type-specific radioimmunoassays for the gp70s of mink cell focus-inducing murine leukemia viruses: expression of a cross-reacting antigen in cells infected with the Friend strain of the spleen focus-forming virus. Journal of Experimental Medicine 148(3): 654-663, 1978