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Hetero transplantation and clonal growth of human philadelphia chromosome positive leukemia cell nalm 1 and bone marrow derived cell leukemia cell balm 2 lines



Hetero transplantation and clonal growth of human philadelphia chromosome positive leukemia cell nalm 1 and bone marrow derived cell leukemia cell balm 2 lines



Cancer 45(9): 2324-2329



Human hematopoietic cell lines, NALM-1, derived from Ph'-chromosome-positive chronic myelocytic leukemia (CML) in blastic crisis, and BALM-2, derived from B[bone marrow-derived]-cell acute lymphoblastic leukemia (ALL), were tested for their heterotransplantability and clonability. Two previously established cell lines, a Burkitt's lymphoma cell line, B35M, and a B-lymphoblastoid cell line, B411-4, derived from a hematologically normal person, were used for comparison. In order for the BALM-2 cell line to grow with 74% success in the immunosuppressed mice, 107 cells were needed, but 108 cells were needed in order for the NALM-1 cell line to grow with 74% success in those mice. As in the case of the BALM-2 line, 107 cells of the B35M cell line were needed for 100% success in heterotransplantation, whereas 3 .times. 107 cells of the B411-4 cell line resulted in 20% success of heterotransplantation. In a semisolid agarose (0.15%) medium, the cloning efficiencies of BALM-2, B35M and B411-4 were 1.52-2.08, 26.31-38.46, and 0.09-0.12, respectively. With an many as 25,000 cells per well plated, NALM-1 did not produce any colonies in the semisolid agarose medium. The heterotransplantability and clonability of cultured cells may not be invariably reliable characteristics of all proven human leukemia-lymphoma cell lines. The behavior of these leukemia cell lines representing their respective original leukemia clones is interpreted as reflecting the heterogeneity inherent in the biologic characteristics seen in human leukemias.

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