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Heterogeneity of human lymphocyte fc receptors part 2 relationship to antibody dependent cell mediated cyto toxicity



Heterogeneity of human lymphocyte fc receptors part 2 relationship to antibody dependent cell mediated cyto toxicity



Clinical and Experimental Immunology 34(2): 274-280



Human peripheral blood lymphocyte (PBL) were incubated (stripped) with Pronase or papain and compared with unstripped lymphocytes for their ability to mediate antibody-dependent, cell-mediated cytotoxicity (ADCC). Despite marked removal or inactivation of receptors for heat-aggregated Ig[immunoglobulin]G (aggG) by proteolytic digestion, and pronounced changes in the percentages of cells rosetting with IgG-sensitized erythrocytes (EA) (decreased by papain, increased by Pronase), stripped PBL functioned normally in ADCC. Stripped and unstripped lymphocytes were pre-treated with aggG to determine the role of aggG receptors in ADCC. AggG almost totally abolished ADCC by unstripped PBL, but inhibited ADCC by enzyme-stripped lymphocytes relatively poorly. Neither untreated nor stripped PBL were able to induce cytotoxicity of chicken erythrocyte (CRBC) target cells sensitized with the Fab'2 fragment of anti-CRBC IgG antibody (CRBC-A). Exposure of PBL to EA monolayers composed of CRBC-A or of sheep erythrocytes (SRBC) sensitized with rabbit anti-SRBC IgG antibody (SRBC-A) depleted PBL of cells that rosetted with CRBC-A and with human Rh-positive, type O erythrocytes sensitized with the human anti-Rh serum Ripley (HRBC-A Ripley). Non-adherent cells were incapable of binding aggG and had markedly diminished cytotoxicity in ADCC. Exposure of PBL to HRBC-A Ripley monolayers resulted in non-adherent cells that were incapable of rosette formation with HRBC-A or CRBC-A, failed to bind aggG and exhibited significantly diminished ADCC activity. Cytotoxic effector PBL active in ADCC (K [killer] cells) have receptors for aggG and for EA. PBL deficient in functional aggG receptors (enzymatically inactivated or removed) are capable of inducing normal levels of ADCC. aggG and EA receptors appear to be closely associated on native K cell membranes. There is no clear-cut relationship in a given lymphocyte population between the presence of aggG or EA receptors and ADCC activity. Populations of PBL binding HRBC-A Ripley overlap with, and may be identical to, those binding aggG and other types of EA complexes.

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Accession: 005565957

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