High performance aqueous gel permeation chromatographic analysis of beta limit dextrin hydrolysis by malted hard red spring wheat triticum sativum cultivar neepawa malted durum wheat triticum durum cultivar wascana and fungal aspergillus oryzae alpha amylases

Kruger, J.E.; Marchylo, B.A.

Cereal Chemistry 59(6): 488-492


ISSN/ISBN: 0009-0352
Accession: 005570620

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High-performance aqueous gel permeation chromatography using a Spherogel-TSK 3000 SW column with an exclusion limit of 1.5 .times. 105 daltons was used to analyze the MW distribution of products formed by .alpha.-amylase catalyzed hydrolysis of .beta.-limit dextrin. Products formed by .alpha.-amylases purified from malted hard red spring wheat (T. aestivum L. cv. Neepawa), malted durum wheat (T. durum cv. Wascana) and fungal (A. oryzae) sources were studied and compared. The breakdown of .beta.-limit dextrin proceeded in stages. Initially, the .beta.-limit dextrin solution exhibited a loss in turbidity concomitant with rapid production of high MW dextrin products larger than 1.5 .times. 105 daltons and little production of low MW dextrins and sugars. This was followed by formation of intermediate MW products that slowly decreased in size concurrent with a progressive buildup of low MW sugars and oligosaccharides. Malted hard red spring and durum wheat .alpha.-amylase hydrolysis appeared identical but differed from that effected by fungal .alpha.-amylase.