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High performance liquid chromatographic method using fluorescence detection for quantitative analysis of zearalenone and alpha zearalenol in blood plasma



High performance liquid chromatographic method using fluorescence detection for quantitative analysis of zearalenone and alpha zearalenol in blood plasma



Journal of the Association of Official Analytical Chemists 64(2): 302-310



A sensitive, high performance liquid chromatographic method was described for quantitative determination of zearalenone [a Fusarium mycotoxin] and .alpha.-zearalenol in blood plasma. Blood plasma was extracted with 2-propanol in ether, the extract evaporated to dryness and the residue dissolved in 0.18 N NaOH. The aqueous phase was washed with chloroform, dichloromethane and benzene, neutralized with 0.10 M H3PO4 and extracted with benzene. The extract was evaporated, dissolved in methanol and injected onto a reverse phase column containing LiChrosorb RP-8 under the following conditions: methanol-acetonitrile-water mobile phase, fluorescence detector, excitation wavelength 236 nm and 418 nm cut-off emission filter. The limit of detectability (twice background) was 0.5 ng standard which was equivalent to 0.6 ng standard/ml blood plasma. Linear standard curves were observed over the range of 0-35 ng of injected zearalenone and .alpha.-zearalenol. Recoveries from blood plasma were 76-101% in the range of 1.5-6.0 ng standard/ml blood.

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Accession: 005571340

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PMID: 6453116



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