High-performance liquid chromatography detection of morphine by fluorescence after post-column derivatisation
Nelson, P.E.; Nolan, S.L.; Bedford, K.R.
Journal of Chromatography 234(2): 407-414
1982
ISSN/ISBN: 0021-9673 PMID: 7056832 Accession: 005571550
A reversed-phase high-performance liquid chromatographic [HPLC] method for the fluorometric determination of morphine in biological samples was developed. Column effluent was mixed with alkaline potassium ferricyanide to produce the fluorescent dimer pseudomorphine. The method provided higher chromatographic specificity and sensitivity than conventional HPLC. Morphine was detected at levels of 10 ng on column.
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