High pressure liquid chromatographic assay of cefamandole in serum following intra venous and intra peritoneal administration
Bawdon, R.E.; Leveno, K.J.; Quirk, J.G.; Cunningham, F.G.; Guss, S.P.
Journal of Liquid Chromatography 6(14): 2747-2760
ISSN/ISBN: 0148-3919 Accession: 005572181
Following cesarean section 102 women were treated with cefamandole by perioperative i.v. administration or i.p. irrigation. High-pressure liquid chromatographic (HPLC) methods for the quantitation of the low serum levels of cefamandole following i.p. lavage were developed. The antibiotic was assayed in the serum using a standard microbiological assay [Bacillus subtilis] and 2 types of reverse phase column technology for HPLC. The 2 HPLC systems were almost identical in performance. HPLC methods were at least 10-fold more sensitive than the microbiological assay. The correlation between the 3 methods was 0.9739. The half-life of cefamandole was 37 min, which was not significantly different from the half-life of the drug in serum of nonpregnant women. The peak serum levels were 47.6 .+-. 36.8 .mu.g/ml and 1.98 .+-. 1.5 .mu.g/ml for the i.v. and i.p. methods of administration, respectively.