High pressure liquid chromatographic determination of beta-sitosterol and beta-sitosterol-d-glucoside in whiskey Spirits, composition, methods, chemical analysis, chromatography
Byrne, K.J.; Reazin, G.H.; Andreasen, A.A.
Journal - Association of Official Analytical Chemists 64(1): 181-185
ISSN/ISBN: 0004-5756 Accession: 005572223
A high pressure liquid chromatographic method is presented for determining the oak wood steroids, .beta.-sitosterol and .beta.-sitosterol-D-glucoside, which contribute to floc formation in whisky. The steroids are extracted from whisky by using an adsorbent resin, Amberlite XAD-2; they are detected at 210 nm, or are derivatized to 2,5-dinitrobenzoate esters, which are detectable over a wide UV wavelength range. Detection limits were less than 0.1 ppm for .beta.-sitosterol, and less than 0.2 ppm for .beta.-sitosterol-D-glucoside in most whiskies, using the extraction procedure described. When the steroids were derivatized, the detection limits were decreased by about a factor of 10.