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Histo compatibility typing by cyto toxic lymphocytes the hla a 2 28 hla b 5 w 35 serologically cross reactive groups reviewed by cyto toxic lymphocytes



Histo compatibility typing by cyto toxic lymphocytes the hla a 2 28 hla b 5 w 35 serologically cross reactive groups reviewed by cyto toxic lymphocytes



Tissue Antigens 17(5): 464-472



Using a standardized indirect cell-mediated lympholysis (CML) technique, cytotoxic lymphocytes (CTL) were raised between unrelated, fully HLA-A, B (and C) typed [human] individuals, differing only for 1 identified HLA-A, B antigenic determinant. Ten CTL involved HLA-A2+ stimulators and HLA-A2-, A28-, A9-responders. Five CTL involved HLA-Bw35+ stimulators and HLA-B5-, Bw35-, B15-, B17-, B18- and Bw21-responders. These CTL were tested for cell-mediated cytotoxicity against 2 different panels (20 and 9 cells, respectively) of HLA-A28+ and HLA-B5+ PHA[phytohemagglutinin]-lymphoblasts. The same panel cells were used as cold competitors for cytotoxicity against the specific 51Cr labeled targets. Most panel cells are neither lysed nor able to block; significant lysis is seen in the panels positive for serologically cross-reactive antigens, although lysis is generally lower than comparable lysis by anti-A28 or anti-B5 CTL. All target cells lysed in the A28 panel can inhibit specific lysis; 2 cells in the B5 panel are lysed but do not inhibit (Cytotoxicity-Positive-Inhibition-Negative = CYPIN). A group of panel cells cannot be lysed but can inhibit (Cytotoxicity-Negative-Inhibition-Positive = CYNIP). Cross-reactive groups identified by serology apparently may be identified by cellology using direct cytotoxicity and/or cold target inhibition. The cross-reactivity defined by CTL, as in serology, is inconstant and often incomplete. Whether the immunogenetic background of cross-reactivity as defined by antibody or CTL is identical is unknown but probable. The occurrence of CYNIP underlines that direct cytotoxicity and cold target inhibition are not directly comparable and indicates that different epitopes may be involved, although the discrepancy may be brought about by the experimental conditions used. The CYPIN phenomenon indicates the existence of immunodominant epitopes although non-T cell-mediated lysis cannot be excluded.

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Accession: 005575644

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