+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Histone variants and histone modifications in chromatin fractions from hetero chromatin rich peromyscus eremicus cells

Histone variants and histone modifications in chromatin fractions from hetero chromatin rich peromyscus eremicus cells

Experimental Cell Research 138(2): 271-286

The relationship between condensed heterochromatin and histone modification by acetylation, phosphorylation and amino acid variation was investigated. Chromatin from cultured P. eremicus cells, containing 35% constitutive heterochromatin, was fractionated into heterochromatin-enriched and depleted fractions. The constitutive heterochromatin content of these fractions was determined from satellite DNA content. The distribution of phosphorylated and acetylated histones and amino acid variants of histone H2A in these chromatin fractions was examined by gel electrophoresis. Fractionation of histones demonstrated that endogenous histone phosphatase activity was high in chromatin fractions and could not be inhibited sufficiently to allow accurate histone phosphorylation measurements. Sodium butyrate did inhibit deacetylation activity in the fractions, allowing histone acetylation measurements to be made. The constitutive heterochromatin content of these fractions was proportional to both their unacetylated H4 content and their more hydrophobic H2A content. These observations support, by direct measurement, earlier experiments suggesting that constitutive heterochromatin is enriched in unacetylated arginine-rich histones and in the more hydrophobic variant of histone H2A.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 005581050

Download citation: RISBibTeXText

Related references

Histone variants and histone modifications in chromatin fractions from heterochromatin-rich Peromyscus cells. Experimental Cell Research 138(2): 271-285, 1982

C banding of peromyscus eremicus constitutive hetero chromatin persists following histone hyper acetylation. Experimental Cell Research 147(2): 269-280, 1983

Histone modifications and hetero chromatin content of cultured peromyscus cells. Federation Proceedings 39(6): ABSTRACT 2119, 1980

Histone variants histone modifications and chromatin structure. Sarma, R H (Ed ) Biomolecular Stereodynamics, Vol 2 Meeting, Albany, N Y , Usa, April 26-29, Xiii+501p Adenine Press: Guilderland, N Y , Usa Illus P125-144, 1981

Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. Histone H1 in chromatin and in H1 - DNA complexes. European Journal of Biochemistry 57(1): 97-105, 1975

Influence of histone kinase phosphorylating lysine rich histone fractions on the physicochemical properties of the chromatin of hepatocytes in the normal state and after partial hepatectomy. Molecular Biology (English Translation of Molekulyarnaya Biologiya (Moscow)) 11(3 PART 2): 550-554, 1977

Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications. Csh Protocols 2007: Pdb.Prot4767, 2007

Hetero chromatin markers arrangement of obligatory hetero chromatin histone genes and multi site gene families in the interphase nucleus of drosophila melanogaster. Chromosoma 86(4): 443-456, 1982

Nucleosomes of active chromatin from sea urchin embryo cells are rich in early histone variants. Molekuliarnaia Biologiia 22(1): 257-266, 1988

Structure of eukaryotic chromosomes and chromatin part 1 histone interactions histone modifications and chromatin structure. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 291-294, 1978

Preparation of eu chromatin rich and hetero chromatin rich fractions by divalent cation dependent auto digestion. Journal of Cell Biology 67(2 PART 2): 326A, 1975

Structure of Eukaryotic Chromosomes and Chromatin || Histone Interactions, Histone Modifications and Chromatin Structure. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 283(997): 291-293, 1978

Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods in Molecular Biology 538: 409-423, 2009

Inference of interactions between chromatin modifiers and histone modifications: from ChIP-Seq data to chromatin-signaling. Nucleic Acids Research 42(22): 13689-13695, 2015

Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. Genes & Development 15(21): 2837-2851, 2001