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Hormonal control of mitotic development in tobacco nicotiana tabacum cultivar maryland protoplasts 2 dimensional distribution of newly synthesized proteins



Hormonal control of mitotic development in tobacco nicotiana tabacum cultivar maryland protoplasts 2 dimensional distribution of newly synthesized proteins



Plant Physiology 68(6): 1273-1278



Two-dimensional separation of proteins newly synthesized by tobacco mesophyll protoplasts cultivated in vitro allows reproducible detection of 257 spots. The pattern is extremely stable throughout the 3 days of culture; the intensity of only 24 spots varied during this time. The absence of cytokinin (N6-benzyladenine) in the culture medium prohibits entry into S phase but does not modify the pattern, indicating that none of the observed proteins is specifically synthesized in S, G2 or M phases. The presence of 2,4D is necessary for the mitotic development of protoplasts. It induces the appearance of one protein, increases the level of another, and reduces that of 8 others. All proteins sensitive to auxin belong to the group of proteins the levels of which vary during culture.

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Accession: 005585930

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