Human alpha1-acid glycoprotein: purification from urine proteins and immunological estimation of its serum concentration
Shibata, K.; Okubo, H.; Ishibashi, H.; Kawamura, K.
Biochemical Medicine 13(3): 251-260
ISSN/ISBN: 0006-2944 PMID: 812491 Accession: 005590292
Human .alpha.1-acid glycoprotein was isolated from urine proteins of a patient with nephrotic syndrome. The isolated preparation was homogeneous in electrophoretical, immunological and ultracentrifugal analysis and was immunologically identical to serum .alpha.1-acid glycoprotein. A potent antiserum specific to this protein was obtained by immunizing rabbits. Using purified .alpha.1-acid glycoprotein and its prepared antiserum, the concentration of .alpha.1-acid glycoprotein in human serum was determined by a single radial immunodiffusion. The mean value of this glycoprotein in serum was 96.5 .+-. 3.1 mg/dl for normal individuals.