Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src

Anderson, S.K.; Gibbs, C.P.; Tanaka, A.; Kung, H.J.; Fujita, D.J.

Molecular and Cellular Biology 5(5): 1122-1129


ISSN/ISBN: 0270-7306
PMID: 2582238
Accession: 005591094

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The nucleotide sequence of the 3' 2/3 of a highly conserved, molecularly cloned human cellular src gene (c-src) was determined. This region of the c-src gene encodes the tyrosine kinase domain of the cellular src protein (pp60c-src) and corresponds to exons 6 through 12 of the chicken c-src gene, as well as nucleotides 545-1542 of the Rous sarcoma virus src gene (v-src). The human c-src sequence is very strongly conserved with respect to both the chicken c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide homology observed in this region. Amino acid sequence conservation in this region is even greater; 98% of the amino acids are conserved between human and chicken c-src. The exon sizes and the locations of the exon-intron bondaries are identical in the human and chicken c-src genes. Sequences within the introns were not conserved, and the introns within the human c-src gene are significantly larger than the corresponding introns within the chicken c-src gene. The strong amino acid conservation between the carboxy-terminal 2/3 of pp60c-src of species as divergent as humans and chickens suggests that this portion of the pp60c-src protein specifies one or more functional domains that are of great important to some aspect of normal cellular growth or differentiation.