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Human lymphocyte antibody dependent cell mediated cyto toxicity toward human red blood cells



Human lymphocyte antibody dependent cell mediated cyto toxicity toward human red blood cells



Blood 52(4): 696-705



Previous investigations involving mononuclear cell ADCC towards human red cell (RBC) targets indicated that monocytes but not lymphocytes are able to produce erythrocyte lysis. This reported insensitivity of human RBC to lymphocyte cytotoxicity was reexamined using a variety of human RBC targets and a wide range of antisera to various erythrocyte antigens. Normal O+ red cells (R1R2-CD.hivin.e/.hivin.cDE) pretreated with small amounts of typical anti-D serum were quite susceptible to monocyte lysis but were completely resistant to lymphocyte ADCC. Other human RBC-antibody combinations [4] mediated striking lymphocyte and monocyte cytotoxicity. These included -D-cells plus typical anti-D, normal O+ cells (R1R2) plus Ripley anti-CD, papain-pretreated normal O+ cells plus small amounts of anti-D and normal O+ cells plus very large amounts of anti-D. Human RBC are not uniquely resistant to lymphocyte ADCC, and antibody density and/or distribution on the target cell surface may be very important to cytotoxicity by leukocytes. The basic characteristics of lymphocyte ADCC toward human RBC were similar to those reported for nucleated targets. Cytolysis was temperature-dependent and rapid, with nearly all erythrocyte lysis occurring in the first 4 h of incubation. Extent of target cell destruction was positively related to the effector/target ratio and the amount of antibody bound to the erythrocyte surface. Further evidence showing the antibody-dependent cell contact-mediated nature of the cytolytic event includes inhibition of ADCC with nonspecific immunoglobulin and data obtained from innocent bystander studies.

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