Human lymphocyte response to carcino embryonic antigen titration of different carcino embryonic antigen samples and neutralization experiments with mono clonal anti carcino embryonic antigen antibodies

Mueller, M.; Irmscher, J.; Zotter, S.; Heidl, G.; Wagner, H.; Kotzsch, M.; Grossmann, H.

European Journal of Cancer and Clinical Oncology 19(10): 1421-1430


ISSN/ISBN: 0277-5379
Accession: 005593201

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By the macrophage electrophoretic mobility technique a striking digestive system cancer-associated human lymphocyte response to CEA was found during a large-scale study including tests in 499 individuals. The study was conducted to determine whether this response is really CEA-specific. Titration experiments with 3 different CEA preparations in lymphocytes from 5 colorectal cancer patients showed that the threshold dose of CEA necessary to induce lymphocyte responses amounts to 50-100 ng CEA/ml and 106 lymphocytes, regardless of the CEA origin and its state of purity. The CEA specificity of the responses was proven by neutralization experiments with 3 CEA-specific monoclonal antibodies. When allowed to react with CEA before lymphocyte incubation, the MAB prevented CEA from inducing lymphocyte responses. Appropriate murine control myeloma protein did not influence these responses. The reactivity of these lymphocyte samples to a teratocarcinoma extract could not be prevented by treating this material with CEA-specific MAB before incubation. Preliminary attempts to enrich the lymphokine(s) released after CEA stimulation resulted in recovery of the activity within 2 arbitrarily cut Sephadex G-100 fractions comprising the MW range of 3000-47,000.