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Humoral immune responses to soluble somatic extracts of Dipetalonema viteae adult worms infected hamsters

Humoral immune responses to soluble somatic extracts of Dipetalonema viteae adult worms infected hamsters

Southeast Asian Journal of Tropical Medicine and Public Health 16(1): 22-30

The present study examined the serum IgG1 and IgM antibody responses of infected hamsters to unfractionated and fractionated soluble somatic (SS) antigens of adult D. viteae. Male and female adult worms were homogenized in a French pressure cell and the SS proteins extracted with 0.375 M Tris-glycine buffer. Serum IgG1 and IgM antibody responses in D. viteae infected with LVG, PD4 and CB hamster strains to the male and female unfractionated SS proteins were measured by an indirect enzyme linked immunosorbent assay (ELISA). Serum IgG1 antibody responses to the SS proteins were similar in all these strains of hamsters during the course of infection. There was no correlation between the level of IgG1 antibody and the onset of microfilariae clearance. The serum IgM antibody response was similar in both outbred LVG and inbred PD4 hamster strains. A lower IgM antibody response was found in CB hamsters and could be related to the failure of this hamster strain to eliminate microfilariae. The SS proteins of male and female adult worms were fractionated by preparative flat-bed isoelectric focusing on granulated gels (PIEF) to yield 8 and 7 fractions, respectively. The comparative antigenicity of the PIEF fractions for the SS proteins was measured by ELISA, using hyperimmune serum from LVG hamsters and rabbit antihamster IgG1- and IgM-alkaline phosphatase conjugates. No difference in ELISA reactivity was noted among the 8 and 7 PIEF fractions from male and female SS proteins. Immunodiffusion studies and sodium dodecyl sulfate polyacrylamide gradient gel electrophoresis (SDS-PAGE) patterns demonstrated a significant heterogeneity and cross reaction of component proteins among the PIEF fractions of each parasite extract.

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Accession: 005596315

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PMID: 4040654

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