Identification of cyto kinins associated with mitosis in synchronously cultured tobacco nicotiana tabacum cultivar xanthi cells

Nishinari, N.; Syono, K.

Plant and Cell Physiology 21(3): 383-394


ISSN/ISBN: 0032-0781
Accession: 005612737

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The amount of endogenous cytokinins in the butanol-soluble fraction increased about 5 times during the mitosis of synchronously dividing cultured tobacco cells (N. tabacum cv. Xanthi). The free cytokinins were identified as trans-zeatin and zeatin ribonucleoside by paper and thin layer chromatography, and by high performance liquid chromatography combined with mass spectrometry and bioassay. The water-soluble fraction contained zeatin ribonucleotide, isopentenyladenosine monophosphate, phosphoglucosylzeatin and phosphoglucosylzeatin ribonucleoside. Changes in the cytokinins in the water-soluble and tRNA fractions during the cell cycle did not support the hypothesis that they are the source of butanol-soluble cytokinins which increased during mitosis. These seem to be synthesized de novo during mitosis.