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Identification of ribophorins in rough microsomal membranes from different organs of several species


Identification of ribophorins in rough microsomal membranes from different organs of several species



European Journal of Biochemistry 124(1): 217-222



ISSN/ISBN: 0014-2956

PMID: 7084228

DOI: 10.1111/j.1432-1033.1982.tb05928.x

Microsomes prepared from several animal sources were analyzed for the presence of proteins corresponding to the ribophorins (I and II) which were previously characterized in rat liver rough microsomes and appear to be involved in the binding of polysomes to endoplasmic reticulum membranes. In rough microsomal membranes from rat lacrimal gland, rabbit liver, dog and chicken pancreas, and mouse myeloma, ribophorin-like polypeptides with similar electrophoretic mobilities were detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis [SDS-PAGE]. In all cases the polypeptides remained associated with sedimentable polysomes after solubilization of the microsomal membranes with nonionic detergents. Ribophorin-like polypeptides were absent from smooth microsomes. Antibodies raised against each rat liver ribophorin, purified by preparative SDS-PAGE, immunoprecipitated only the corresponding polypeptide, indicating no crossreactivity between ribophorins I and II. These antibodies also immunoprecipitated the homologous ribophorins found in microsomal preparations from other organs and species.

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Accession: 005615107

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Related references

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