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Immuno diagnosis of infection with schistosoma mansoni comparison of enzyme linked immuno sorbent assay radio immunoassay and precipitation tests performed with antigens from eggs



Immuno diagnosis of infection with schistosoma mansoni comparison of enzyme linked immuno sorbent assay radio immunoassay and precipitation tests performed with antigens from eggs



American Journal of Tropical Medicine & Hygiene 28(4): 661-669



The circumoval precipitin (COP) test with fresh whole eggs of S. mansoni was more sensitive and specific than other tests with cercariae or adult worms or their extracts as antigens [Ag]. More current serologic tests were evaluated in which eggs or soluble extracts of eggs were used as Ag. Serum samples from 121 [human] individuals who participated in a longitudinal population-based study of schistosomiasis in Parcelas de Boqueron, Puerto Rico, were used in this study. All serum samples were tested blindly for antibodies to S. mansoni by the various serologic tests. Infection with S. mansoni was determined by examining a series of fecal samples (3-6) by the modified Ritchie formol-ether concentration technique. The radioimmunoassay with MSA1 as Ag and the COP test had a diagnostic sensitivity of 95% and detected 100% of the individuals excreting 10 or more eggs/g. The enzyme-linked immunosorbent assay (ELISA) with soluble egg Ag had a sensitivity of only 75% and the Ouchterlony immunodiffusion with the same Ag only 41%. Specificity was highest with the COP test, which correctly identified 96% of the uninfected individuals. A rating index was useful in ranking these tests according to their diagnostic strength and in establishing a criterion of positivity for the ELISA.

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