Immuno fluorescence observation of the infection of densonucleosis virus in the silkworm bombyx mori
Maeda, S.; Watanabe, H.
Japanese Journal of Applied Entomology and Zoology 22(2): 98-101
ISSN/ISBN: 0021-4914 Accession: 005624098
The infection and multiplication site of a densonucleosis virus (Densovirus) in the silkworm, Bombyx mori, were studied by the fluorescent antibody technique. Among various tissues of infected larvae, only the midgut epithelium showed specific fluorescence. The fluorescence was restricted to the nuclei of the columnar cells, showing the site of virus multiplication. The fluorescence appeared sporadically in a few columnar cells 24 h after virus administration and became more intense and increased in number with time. At 72 h after virus administration, most of the columnar cells showed fluorescence. However, a few columnar cells near the cardiac valve did not show any fluorescence even in the later stages of the disease.