Immuno gold localization of alpha l arabinofuranosyl residues in pollen tubes of nicotiana alata link et otto
Anderson, M.A.; Harris, P.J.; Bonig, I.; Clarke, A.E.
Planta (Heidelberg) 171(4): 438-442
Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for .alpha.-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall; the inner, non-fibrillar wall layer was not labelled. Cytoplasmic vesicles (0.2 .mu.m diameter) were also labelled. The antibody may bind to an arabinan in the pollen-tube wall.