Section 6
Chapter 5,626

Immuno gold localization of alpha l arabinofuranosyl residues in pollen tubes of nicotiana alata link et otto

Anderson, M.A.; Harris, P.J.; Bonig, I.; Clarke, A.E.

Planta (Heidelberg) 171(4): 438-442


ISSN/ISBN: 0032-0935
Accession: 005625623

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Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for .alpha.-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall; the inner, non-fibrillar wall layer was not labelled. Cytoplasmic vesicles (0.2 .mu.m diameter) were also labelled. The antibody may bind to an arabinan in the pollen-tube wall.

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