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Immuno gold localization of nitrate reductase in spinach spinacia oleracea leaves


Immuno gold localization of nitrate reductase in spinach spinacia oleracea leaves



Plant and Cell Physiology 28(2): 333-338



ISSN/ISBN: 0032-0781

The intracellular location of nitrate reductase in spinach leaves was examined by applying an immunocytochemical method. Thin sections were first treated with immunopurified anti-nitrate reductase monospecific antibodies, followed by incubation with colloidal gold-labelled goat anti-rabbit immunoglobulin G as a marker. The nitrate reductase was specifically located in the chloroplast. When anti-nitrate reductase antibodies were omitted, or when pre-immune serum was used no label was observed.

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Accession: 005625627

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