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Immunogenic variants obtained by mutagenesis by mouse masto cytoma p 815 7. dominant expression of variant antigens in somatic cell hybrids



Immunogenic variants obtained by mutagenesis by mouse masto cytoma p 815 7. dominant expression of variant antigens in somatic cell hybrids



Somatic Cell Genetics 9(3): 345-358



After mutagenesis of mouse mastocytoma P815, it is possible to obtain at high frequency stable tumor cell variants (tum-) that are rejected by syngeneic DBA/2 mice. Most of the variants express one or more new individual antigens specific for each variant, that are detectable in vitro by cytolytic T cells (CTL). Somatic hybrids were prepared either between tum- variants and the original P815 clone, or between different variants. Antigen expression of the hybrids was assessed by using long-term CTL clones that recognize specifically the new antigen present on the variants. Expression of tum- variant antigens behaved as a dominant trait in the hybrids. By submitting the somatic hybrids to selection with CTL clones, it was possible to obtain antigen-loss hybrid variants. The analyses of these antigen-loss variants showed that 2 variant-specific antigenic determinants associated with one of the variant fusion partners could be lost independently. Like the parental tum- variants, both the (tum+ .times. tum-) and (tum- .times. tum-) hybrids failed to form tumors in normal mice but formed tumors in irradiated mice.

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