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Immunogenic variants obtained by mutagenesis of mouse masto cytoma p 815 1. rejection by syngeneic mice



Immunogenic variants obtained by mutagenesis of mouse masto cytoma p 815 1. rejection by syngeneic mice



Journal of Experimental Medicine 152(5): 1175-1183



It is possible to obtain variants that are incapable of forming progressive tumors in syngeneic mice (tum-) by mutagenesis and cloning of a teratocarcinoma and a Lewis lung carcinoma cell line. These observations were extended to the ascitic P815 mastocytoma cells of mouse strain DBA/2. After treatment with the mutagen N-methyl-N'-nitro-N-nitrosoguanidine, a high frequency (14%) of tum- clones were obtained. A colony assay indicated that after a period of rapid multiplication extending to .apprx. 10 days after injection, the P815 tum- cells were rejected by a process that was usually completed by day 15. No rejection was observed in sublethally irradiated animals. The immunological nature of the rejection of the P815 variants was further inferred because, upon rejection, the mice acquired a radioresistant specific protection that could be transferred adoptively with spleen cells. Cross-immunization patterns demonstrated the presence of singular antigenic specificities on 3 of the 5 variants examined. A common antigen was found on all the tum- variants and the original cells capable of forming progressive tumors in syngeneic mice (tum+). Mice injected with tum- cells were significantly protected against a tum+ challenge, even though no significant protection was generated by irradiated tum+ cells.

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Accession: 005630563

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PMID: 6776226


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