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Immunological study of hydatidosis part 1 evaluation of immuno electro diffusion tests and enzyme linked immuno electro diffusion assay in human hydatidosis



Immunological study of hydatidosis part 1 evaluation of immuno electro diffusion tests and enzyme linked immuno electro diffusion assay in human hydatidosis



American Journal of Tropical Medicine & Hygiene 28(2): 318-324



The comparative sensitivity, specificity and rapidity of immunoelectrodiffusion (IED) on cellulose acetate membranes and of immunoelectrophoresis (IEP) on agarose gel were evaluated in the diagnosis of hydatidosis. Pooled crude fluid from several cattle hydatid cysts was used as antigen [Ag] in tests on 1750 non-hydatid and 400 hydatid sera obtained from patients with hepatic, pulmonary, splenic, peritoneal and cerebral hydatidosis before and after surgery. Coupled to a specific human reference serum for arc 5, IED shows a specificity comparable to that of IEP but its sensitivity is slightly higher and the amount of Ag needed is very small. The appearance of a typical gloved finger pattern in sera from patients with ruptured cysts emphasizes the significance of quick results (3 h) obtained by this method. To increase the sensitivity of IED and to define the class of immunoglobulins (Ig) involved in the Ag-antibody [Ab] reaction, this method was coupled with an enzymatic technique. The immune complexes precipitated by IED were treated with peroxidase-labeled Ab specific to each class of human Ig. This enzyme-linked immunoelectrodiffusion assay (ELIEDA) can be used to follow the immunologic evolution of hydatidosis and to identify IgM in ruptured hepatic cysts and IgA in pulmonary cysts.

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Accession: 005635516

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