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Improved high performance liquid chromatographic method for the determination of 6 n n n trimethyllysine in plasma and urine biomedical application of chromatographic figures of merit and amine mobile phase modifiers



Improved high performance liquid chromatographic method for the determination of 6 n n n trimethyllysine in plasma and urine biomedical application of chromatographic figures of merit and amine mobile phase modifiers



Journal of Chromatography Biomedical Applications 380(2): 285-300



An internally standardized method for the determination of 6-N,N,N-trimethyllysine in human plasma, human urine, rat plasma, rat urine and hydrolyzed rat urine is described. This methylated amino acid and the procedural internal standard 6-N,N,N-triethyllysine were isolated from the sample matrices using short ion-exchange columns and detected following high-performance liquid chromatography using a postcolumn reaction (o-phthalic-dicarboxaldehyde-2-mercaptoethanol) and fluorometric detection. The reliable detection limit for 6-N,N,N-trimethyllysine was 0.2 nmol/ml in 200 .mu.l of human plasma The chromatographic separation exploits the unique properties of a novel teritary amine mobile phase modifier, 3-(N,N-dimethylamino)-1,2-propanediol. The capacity factor and "Chromatographic Figures of Merit" (including peak asymmetry and relative system efficiency) were calculated for the chromatographic peak representing 6-N,N,N-trimethyllysine in over 2200 injections made while evaluating 900 biological specimens.

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Accession: 005641563

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PMID: 3093513


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