+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro adhesion of k88ab positive k88ac positive and k88ad positive escherichia coli to intestinal villi to buccal cells and to erythrocytes of weaned piglets



In vitro adhesion of k88ab positive k88ac positive and k88ad positive escherichia coli to intestinal villi to buccal cells and to erythrocytes of weaned piglets



Veterinary Microbiology 15(3): 201-208



The phenotype of 21 weaned piglets, concerning adhesion of Escherichia coli possessing K88ab, K88ac or K88ad fimbriae to pig cells, were determined in an in vitro assay. Comparison was made with adhesion of these three K88 variant strains to buccal mucosal epithelial cells and to erythrocytes (haemagglutination) in the same piglets. Whereas adhesion of the three K88 variant strains to intestinal villi was piglet specific, buccal cell adhesion (BCA) and haemagglutination (HA) were not. The K88ab strain was weakly adhesive or non-adhesive in the BCA and negative in the HA test. K88ac strains consistently gave negative and K88ad consistently gave positive results in both assays. After washing the bacteria with phosphate-buffered saline, the K88ab strain revealed a positive HA test. Neither the BCA, nor HA test can be used to determine the pig intestinal adhesive phenotype.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005645704

Download citation: RISBibTeXText


Related references

In vitro adhesion of K88ab-, K88ac- and K88ad-positive Escherichia coli to intestinal villi, to buccal cells and to erythrocytes of weaned piglets. Veterinary Microbiology 15(3): 201-207, 1987

In vitro adhesion of K88 positive Escherichia coli to intestinal villi of just-weaned piglets. New developments in biosciences: their implications for laboratory animal science: proceedings of the Third Symposium Amsterdam The Nethrlands 1-5 June 1987 edited by Anton C Beyneen and Henk A Solleveld: 397, 1988

In vitro adhesion of k88ac positive escherichia coli to peyer's patch and peripheral blood lymphocytes buccal and rectal epithelial cells or intestinal epithelial brush borders of weaned pigs. Veterinary Microbiology 20(4): 357-368, 1989

Effect of experimentally-induced villus atrophy on adhesion of K88ac-positive Escherichia coli in just-weaned piglets. Veterinary Microbiology 17(2): 159-169, 1988

PCR assay for detection and differentiation of K88ab(1), K88ab(2), K88ac, and K88ad fimbrial adhesins in E. coli strains isolated from diarrheic piglets. Folia Microbiologica 50(2): 107-112, 2005

Electron microscopic study of an experimental combined infection of just-weaned piglets with transmissible gastroenteritis virus and K88ac positive enterotoxigenic Escherichia coli. Zentralblatt für Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B 35(5): 317-330, 1988

In vitro adhesion of K88ac+Escherichia coli to Peyer's patch and peripheral blood lymphocytes, buccal and rectal epithelial cells or intestinal epithelial brush borders of weaned pigs. Veterinary Microbiology 20(4): 357-368, 1989

Comparison of the in vitro adhesion of K88, K99, F41 and P987 positive Escherichia coli to intestinal villi of 4- to 5-week-old pigs. Veterinary Microbiology 34(1): 7-18, 1993

Monoclonal antibodies specific for K88ab, K88ac and K88ad antigens of Escherichia coli. Wei Sheng Wu Xue Bao 29(5): 348-353, 1989

Monoclonal antibodies to K88ab, K88ac and K88ad fimbriae from enterotoxigenic Escherichia coli. Microbial Pathogenesis 1(1): 57-69, 1986

Adhesion in vitro of Escherichia coli type K88 to intestinal villi from newly-weaned piglets of a Belgian pig herd. Vlaams Diergeneeskundig Tijdschrift 57(2): 90-96, 1988

Behavior of Escherichia coli K antigens K88ab, K88ac, and K88ad in immunoelectrophoresis, double diffusion, and hemagglutination. Infection and Immunity 23(3): 700-705, 1979

Different pig phenotypes affect adherence of Escherichia coli to jejunal brush borders by K88ab, K88ac, or K88ad antigen. Infection and Immunity 37(3): 891-894, 1982

Characterization of the binding specificity of K88ac and K88ad fimbriae of enterotoxigenic Escherichia coli by constructing K88ac/K88ad chimeric FaeG major subunits. Infection and Immunity 77(2): 699-706, 2008

Characterization of fimbrial and hemolysin genes from a pap positive sfa positive pil positive hly positive pyelonephritogenic escherichia coli. Abstracts of the General Meeting of the American Society for Microbiology 91: 32, 1991