Section 6
Chapter 5,646

In vitro analysis of pre implantation and early post implantation development of lethal yellow a y a y mouse embryos/

Johnson, L.L.; Granholm, N.H.

Journal of Experimental Zoology 204(3): 381-390


ISSN/ISBN: 0022-104X
Accession: 005645814

Download citation:  

Preimplantation embryos from matings between yellow heterozygous (Ay/a) mice were recovered at 56 h post coitum, cultured for 5 days and compared with the development of embryos from 3 control matings (Ay/a.female. .times. a/a.male., a/a.female. .times. Ay/a.male., a/a.female. .times. a/a.male.). Most embryos were at the 8-cell stage at recovery; however, fewer embryos from the experimental cross had developed to the 8-cell stage than embryos of control matings, indicating a developmental lag of experimental embryos (P < 0.01). The yellow (Ay/a) uterus did not contribute (P = 0.05) to delayed development. Experimental and control embryos were equally capable of successful development in culture to the morula stage with no distinct morphological characteristics identifying the class of Ay/Ay mutants. However, significant differences were observed in the development from morulae to blastocysts; 9.4% (10/106) of the morulae in experimental crosses failed to undergo blastocyst formation as compared with 2.5% (10/398) of morulae in pooled control crosses (P = 0.010-0.025). In the experimental cross 25.0% (24/96) of embryos that developed successfully to the blastocyst stage failed to hatch from the zona pellucida; these were presumed to include the class of lethal yellow homozygotes. Abnormalities seen in cultured embryos consisted primarily of blastomere disintegration, blastomere arrest and exclusion and embryo fragmentation.

PDF emailed within 1 workday: $29.90