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In vitro characterization of the ac locus in white clover trifolium repens



In vitro characterization of the ac locus in white clover trifolium repens



Archives of Biochemistry and Biophysics 218(1): 38-45



Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. L-Valine, 2-methylpropanal oxime and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. L-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least 2 steps in these conversions are missing in microsomes from plants of genotype ac ac.

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Accession: 005646943

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PMID: 7149740


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