+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

In vitro cultivation of bloodstream forms of trypanosoma brucei brucei trypanosoma brucei rhodesiense and trypanosoma brucei gambiense



In vitro cultivation of bloodstream forms of trypanosoma brucei brucei trypanosoma brucei rhodesiense and trypanosoma brucei gambiense



Journal of Protozoology 28(4): 470-479



A series of new in vitro systems for the cultivation of bloodstream forms of T. (Trypanozoon) brucei brucei, T. (T.) b. rhodesiense, and T. (T.) b. gambiense was developed. The standard system consists of a feeder layer of fibroblast-like cells derived from embryos of New Zealand white rabbits (REF) or a mountain vole, Microtus montanus (MEF), with HEPES-buffered Minimum Essential Medium (MEM), with Earle's salts, supplemented with 15% inactivated rabbit serum. These 2 and other feeder layers were cross-checked with different sera to test for growth support of bloodstream forms of the 3 trypanosome subspecies studied. Cultures could be initiated with bloodstream forms from mammalian hosts or from cryopreserved stabilates. Metacyclic forms from infected Glossina m. morsitans could also be used as inoculum; they transformed within 6 h to bloodstream forms. Maintenance of cultures and growth properties are described in detail. Experiments were undertaken to confirm that the cultivated bloodstream forms still possess some of the characteristic features of pleomorphic bloodstream populations. Cultivated bloodstream forms were always infective for mice, and a surface coat could be demonstrated by EM. They could also be cyclically transmitted through tsetse flies, and the metacyclic forms from these flies could be brought back into culture. In vitro cloning with single bloodstream forms and metacyclic forms could be achieved with high cloning efficiency. The consumption of glucose and the production of pyruvate and lactate were determined.

(PDF emailed within 1 workday: $29.90)

Accession: 005647214

Download citation: RISBibTeXText


Related references

Quantitation of genetic differences between trypanosoma brucei gambiense trypanosoma brucei rhodesiense and trypanosoma brucei brucei by restriction enzyme analysis of kinetoplast dna. Molecular & Biochemical Parasitology 3(2): 117-131, 1981

Human serum resistance of metacyclic forms of Trypanosoma brucei brucei, T. brucei rhodesiense and T. brucei gambiense. Parasitology Research 73(3): 218-223, 1987

Purine metabolism in the bloodstream forms of trypanosoma brucei gambiense and trypanosoma brucei rhodesiense. Biochimica et Biophysica Acta 719(2): 223-231, 1982

Nonvariant antigens limited to bloodstream forms of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense. Journal of Protozoology 31(4): 541-548, 1984

Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization. Experimental Parasitology 99(3): 123-131, 2002

Trypanosoma brucei brucei, T. brucei gambiense, and T. brucei rhodesiense: common glycoproteins and glycoprotein oligosaccharide heterogeneity identified by lectin affinity blotting and endoglycosidase H treatment. Experimental Parasitology 63(1): 32-41, 1987

Cultivation in a semi defined medium of animal infective forms of trypanosoma brucei trypanosoma equiperdum trypanosoma evansi trypanosoma rhodesiense and trypanosoma gambiense. EMBO (European Molecular Biology Organization) Journal 4(5): 1273-1278, 1985

Enzyme variation in trypanosoma brucei ssp 2. evidence for trypanosoma brucei rhodesiense being a set of variants of trypanosoma brucei brucei. Parasitology 90(1): 89-100, 1985

In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense. Journal of Protozoology, 284: 470-479, 1981

In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, T. gambiense. Journal of protozoology 30 28(4): 470-479, 1981

Effects of intermediates of methionine metabolism and nucleoside analogs on S-adenosylmethionine transport by Trypanosoma brucei brucei and a drug-resistant Trypanosoma brucei rhodesiense. Biochemical Pharmacology 56(1): 95-103, 1998

In situ kinetic characterization of methylthioadenosine transport by the adenosine transporter (P2) of the African Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense. Biochemical Pharmacology 61(4): 449-457, 2001

Differential effects of interferon- on production of trypanosome-derived lymphocyte-triggering factor by Trypanosoma brucei gambiense and Trypanosoma brucei brucei. Journal of Parasitology ust; 90(4): 740-745, 2004

Differential Effects of Interferon-g on Production of Trypanosome-derived Lymphocyte-triggering Factor by Trypanosoma brucei gambiense and Trypanosoma brucei brucei. Journal of Parasitology 90(4): 0-5, 2004

Differential Effects of Interferon- on Production of Trypanosome-Derived Lymphocyte-Triggering Factor by Trypanosoma brucei Gambiense and Trypanosoma brucei brucei. The Journal of Parasitology 90(4): 740-745, 2004