In vitro effects of methotrexate, 5-fluorouracil, cyclophosphamide and vincristine sulphate on the metabolism of induced mammary cancers in rats

de Loecker, W.; van der Schueren, G.

European Journal of Cancer 14(12): 1363-1368


ISSN/ISBN: 0014-2964
PMID: 738340
Accession: 005648206

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In function of concentrations 0.001-100.0 .mu.g methotrexate added to the incubation medium, the [2-14C]glycine incorporation into the proteins of experimental mammary cancer tissue of rats became progressively reduced. Only the highest concentrations of methotrexate reduced the [1-14C].alpha.-aminoisobutyric acid uptake by the cells. In the present in vitro system, 5-fluorouracil only inhibited the [6-3H]thymidine incorporation into DNA without affecting either glycine incorporation or the .alpha.-aminoisobutyric acid uptake. Cyclophosphamide impaired the glycine incorporation into the proteins as well as the thymidine incorporation into DNA in function of increasing amounts added, while the highest concentrations applied resulted in an increased .alpha.-aminoisobutyric acid uptake by the cells. With vincristine sulphate addition to the incubating tumor-slices, glycine incorporation and .alpha.-aminoisobutyric acid uptake were reduced which was particularly noticeable at the highest dose levels.