+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro modulation of prolactin binding to human mammary carcinoma cells by steroid hormones and prolactin

In vitro modulation of prolactin binding to human mammary carcinoma cells by steroid hormones and prolactin

Journal of Clinical Endocrinology and Metabolism 60(6): 1243-1249

This study demonstrates the specific binding of human (h) PRL to mammary carcinoma cells of the newly established line EFM-19. Under saturating conditions, [125I]hPRL bound to these cells with high affinity (Ka = 4.3 X 10(10) M-1) and low capacity (4080 binding sites/cell). In serum-free medium, stimulation of cell proliferation by PRL was found, suggesting a biological role in the growth of human breast cancer. hPRL was more effective in binding to and promoting the growth of EFM-19 cells than ovine PRL or other lactogenic hormones. Up- and down-regulation of [125I]hPRL binding occurred after pretreatment of EFM-19 cell cultures with subphysiological or higher hPRL concentrations, respectively. Physiological concentrations of 17 beta-estradiol or dihydrotestosterone increased the cellular capacity of hPRL binding. Pharmacological concentrations of dihydrotestosterone or physiological concentrations of progesterone reduced the binding of [125I]hPRL. The results provide evidence for complex regulatory mechanisms of PRL binding by human mammary carcinoma cells involving steroid hormones.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005650060

Download citation: RISBibTeXText

PMID: 2987289

DOI: 10.1210/jcem-60-6-1243

Related references

In vitro growth promotion of human mammary carcinoma cells by steroid hormones, tamoxifen, and prolactin. Journal of the National Cancer Institute 73(2): 313-321, 1984

Modulation of prolactin receptors (PRL-R) by lactogenic and steroid hormones in human breast cancer cells in long-term tissue culture (T-47D). Anticancer Research 9(3): 631-636, 1989

Steroid sex hormones and prolactin in postmenopausal women with generalized mammary carcinoma during prolonged dexamethasone treatment. Journal of Endocrinology 73(2): 235-246, 1977

Steroid hormones modulate prolactin binding by cultured porcine granulosa cells. Biochemical & Biophysical Research Communications 91(1): 284-288, 1979

Prolactin receptor expression in monolayer cultures of rabbit mammary epithelial cells: pre- and postpartum [125I]-prolactin binding activity. Journal of Cellular Biochemistry 22(2): 121-130, 1983

Modulation of growth, prostaglandin synthesis, and prolactin-binding in two cultured rat mammary carcinoma cell lines by flurbiprofen. Prostaglandins Leukotrienes and Medicine 20(2): 223-236, 1985

Effect of hormones on dissociation of prolactin from the rabbit mammary gland prolactin receptor. Biochemical Journal 279: 461-465, 1991

Role of steroid hormones and prolactin in canine mammary cancer. Journal of Steroid Biochemistry and Molecular Biology 94(1-3): 181-187, 2005

Sex steroid and human chorionic gonadotropin modulation of in vitro prolactin production by human term decidua. American Journal of Obstetrics & Gynecology 148(4): 461-465, 1984

Human prolactin receptor variants in breast cancer: low ratio of short forms to the long-form human prolactin receptor associated with mammary carcinoma. Cancer Research 64(16): 5677-5682, 2004

Steroid-hormone modulation of prolactin action in the rat mammary gland. Gene regulation by steroid hormones: 58-77, 1980

Stimulatory effects of prolactin and anti prolactin receptor serum on prolactin binding sites in rat liver cells in suspension culture. Biochemical & Biophysical Research Communications 106(1): 243-249, 1982

Human prolactin regulates transfected MMTV LTR-directed gene expression in a human breast-carcinoma cell line through synergistic interaction with steroid hormones. International Journal Of Cancer. 52(6): 928-933, 1992

Effects of hormones on prolactin binding to normal mammary glands and pregnancy-dependent mammary tumors in GR/A female mice near parturition. Journal of Endocrinological Investigation 3(1): 63-66, 1980

Prolactin and prolactin binding sites in human breast cancer cells. Progress in Clinical and Biological Research 142: 109-117, 1984