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In vitro phosphorylation of escherichia coli isocitrate lyase



In vitro phosphorylation of escherichia coli isocitrate lyase



Current Microbiology 15(2): 103-106



The in vitro phosphorylation of isocitrate lyase was demonstrated in partially purified sonic extracts of Escherichia coli. Extracts were incubated with [gamma 32P]-ATP and subsequently analyzed by two-dimensional polyacrylamide gel electrophoresis. Isocitrate lyase was determined to be phosphorylated by autoradiography and Western blot analyses of the gels. Purified isocitrate lyase comigrates with the phosphorylated form of the enzyme; this suggests that the enzyme may become catalytically active concomitant with phosphorylation.

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Accession: 005650315

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